Dietary assessment methodology and reporting in Pacific Island research: a scoping review protocol.

OBJECTIVE: This scoping review aims to identify and describe the methods used to collect and analyze dietary intake data in residents of Pacific Island countries. INTRODUCTION: Dietary assessment explores associations between dietary factors and health outcomes. In regions, including the Pacific Islands, where diet-related, non-communicable diseases are increasing, this is a growing area of research. As this information is used to inform food and nutrition policies and practice, accurate collection, analysis, and interpretation of dietary assessment data relies on robust methods. A greater understanding of how nutrition studies are designed can strengthen the evidence on nutrition and health in Pacific Island settings and inform future research approaches. INCLUSION CRITERIA: The scoping review will consider studies published in peer-reviewed journals, including quantitative and qualitative study designs and gray literature, including government reports, research and technical reports, theses and dissertations that measure and/or assess dietary intake in resident populations of the Pacific Island member countries of the Secretariat of the Pacific Community: American Samoa, Commonwealth of the Northern Mariana Islands, the Cook Islands, the Federated States of Micronesia, French Polynesia, Guam, the Independent State of Papua New Guinea, the Independent State of Samoa, the Kingdom of Tonga, New Caledonia, Niue, Pitcairn Islands, the Republic of Fiji, the Republic of Kiribati, the Republic of the Marshall Islands, the Republic of Nauru, the Republic of Palau, the Republic of Vanuatu, Solomon Islands, Tokelau, Tuvalu, and Wallis and Futuna. METHODS: There will be no time limit and searches will be conducted in PubMed, CINAHL, CABI, Scopus, Cochrane Library, and Web of Science. Results will be limited to English-language articles. Data will be extracted independently by two reviewers into a charting table. Results will be presented graphically and with tables accompanied by a narrative summary.

Low densities of macroplastic debris in the Pitcairn Islands Marine Reserve.

Beaches on Henderson Island, one of the Pitcairn Islands in the central South Pacific, have been reported to have some of the highest standing stocks of stranded plastic recorded. Surveys of floating debris at sea show that this is not related to high densities of plastic in the area. Only 19 macroplastic items were observed during 905 km of at-sea transects (0.5 items·km-2; 135 g·km-2). This low density confirms previous microplastic surveys which show that the Pitcairn group lies west of the South Pacific Gyre. Plastic loads on beaches at other islands in the Pitcairn group are not exceptional. The east coast of Henderson Island appears to be particularly effective at accumulating drifting debris, but many small plastic fragments on these beaches probably result from degradation in situ, so removing macroplastics will reduce the formation of microplastics.

New cycloartane triterpenes from bioactive extract of propolis from Pitcairn Island.

Dichloromethane extract of propolis (DCME) originating from Pitcairn Island demonstrated potent cytotoxicity against triple-negative MDA-MB-231 human breast carcinoma cells. The results from MTT assay showed that DCME inhibits the growth of the cancer cells in a dose- and time-dependent manner and upon the cell growth inhibition propolis extract provoked apoptotic changes in the cell nuclei. A detailed chemical investigation of DCME led to the isolation of four new cycloartane triterpenes (1-4), along with 17 known compounds (5-21). The structures of the new compounds were elucidated by means of extensive analysis of their spectroscopic data and comparison with those reported for their analogues. In vitro antimicrobial activity of new compounds (1-4) along with the DCME against four human pathogens was evaluated. All tested constituents except compound 2 were highly active against Escherichia coli with MIC 64 µg/ml. Compound 1 exhibited high antifungal activity against Candida albicans with potency close to that of the positive control (amphotericin B). The DCME showed very good antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. This is the first study on propolis from Pitcairn Island.

Significant range extensions for two fish species at Pitcairn Island, South Pacific.

A baited videographic survey at Pitcairn Island has significantly extended the known range for two reef-fish species: blue-lined triggerfish Xanthichthys caeruleolineatus and greater amberjack Seriola dumerili, with an additional minor extension noted for giant trevally Caranx ignobilis. This highlights the importance of further research in this remote, poorly studied island group and provides evidence supplementing the understanding of oceanographic patterns in the South Pacific.

The real bounty: marine biodiversity in the Pitcairn Islands.

In 2012 we conducted an integrated ecological assessment of the marine environment of the Pitcairn Islands, which are four of the most remote islands in the world. The islands and atolls (Ducie, Henderson, Oeno, and Pitcairn) are situated in the central South Pacific, halfway between New Zealand and South America. We surveyed algae, corals, mobile invertebrates, and fishes at 97 sites between 5 and 30 m depth, and found 51 new records for algae, 23 for corals, and 15 for fishes. The structure of the ecological communities was correlated with age, isolation, and geomorphology of the four islands. Coral and algal assemblages were significantly different among islands with Ducie having the highest coral cover (56%) and Pitcairn dominated by erect macroalgae (42%). Fish biomass was dominated by top predators at Ducie (62% of total fish biomass) and at Henderson (35%). Herbivorous fishes dominated at Pitcairn, while Oeno showed a balanced fish trophic structure. We found high levels of regional endemism in the fish assemblages across the islands (45%), with the highest level observed at Ducie (56% by number). We conducted the first surveys of the deep habitats around the Pitcairn Islands using drop-cameras at 21 sites from depths of 78 to 1,585 m. We observed 57 fish species from the drop-cams, including rare species such as the false catshark (Pseudotriakis microdon) and several new undescribed species. In addition, we made observations of typically shallow reef sharks and other reef fishes at depths down to 300 m. Our findings highlight the uniqueness and high biodiversity value of the Pitcairn Islands as one of the least impacted in the Pacific, and suggest the need for immediate protection.

The Norfolk Island Eye Study (NIES): rationale, methodology and distribution of ocular biometry (biometry of the bounty).

AIM: To describe the recruitment, ophthalmic examination methods and distribution of ocular biometry of participants in the Norfolk Island Eye Study, who were individuals descended from the English Bounty mutineers and their Polynesian wives. METHODS: All 1,275 permanent residents of Norfolk Island aged over 15 years were invited to participate, including 602 individuals involved in a 2001 cardiovascular disease study. Participants completed a detailed questionnaire and underwent a comprehensive eye assessment including stereo disc and retinal photography, ocular coherence topography and conjunctival autofluorescence assessment. Additionally, blood or saliva was taken for DNA testing. RESULTS: 781 participants aged over 15 years were seen (54% female), comprising 61% of the permanent Island population. 343 people (43.9%) could trace their family history to the Pitcairn Islanders (Norfolk Island Pitcairn Pedigree). Mean anterior chamber depth was 3.32mm, mean axial length (AL) was 23.5mm, and mean central corneal thickness was 546 microns. There were no statistically significant differences in these characteristics between persons with and without Pitcairn Island ancestry. Mean intra-ocular pressure was lower in people with Pitcairn Island ancestry: 15.89mmHg compared to those without Pitcairn Island ancestry 16.49mmHg (P = .007). The mean keratometry value was lower in people with Pitcairn Island ancestry (43.22 vs. 43.52, P = .007). The corneas were flatter in people of Pitcairn ancestry but there was no corresponding difference in AL or refraction. CONCLUSION: Our study population is highly representative of the permanent population of Norfolk Island. Ocular biometry was similar to that of other white populations. Heritability estimates, linkage analysis and genome-wide studies will further elucidate the genetic determinants of chronic ocular diseases in this genetic isolate.

The plant communities and environmental gradients of Pitcairn Island: the significance of invasive species and the need for conservation management.

Quantitative surveys of the vegetation of south-east Polynesian Islands are rarely undertaken owing to time and logistical restrictions; however they are fundamental in determining the conservation status of fragile island ecosystems. The aim of the research was to document quantitatively the vegetation of Pitcairn Island by investigating whether clearly definable plant communities existed on the island, and the underlying environmental gradients influencing these communities. Initially, 10 x 10 m quadrats were taken from all areas of the island, with environmental parameters recorded for each quadrat. The vegetation was then mapped from high altitude vantage points. Two-way indicator species analysis was used to identify distinct plant communities, and canonical correspondence analysis was used to determine the underlying environmental gradients. The vegetation consists of 14 plant communities: four coastal, six forest, two fernland and two scrub communities. Large areas are covered by non-native scrub vegetation, and by monospecific Syzygium jambos (rose-apple) plantations. Less than 30 % of the island is covered by native forest, and these areas are limited to remote valleys. Fernlands also cover large areas, including both eroding areas and ridge tops. Coastal vegetation comprises rock and cliff communities with limited strand vegetation. The major environmental gradient affecting the composition of the plant communities is altitude, but anthropogenic influences also have a large effect, owing to forest clearance and introduced species. The light environment is affected by the canopy species, and determines what ground flora can develop. Identification of distinct plant communities has allowed for a system of nature reserves to be suggested, which conserve all of these plant communities and a significant proportion of the threatened plant species.

Pitcairn Island: fertility and population growth, 1790-1856.

The hybrid English-Tahitian community which lived on Pitcairn Island between 1790 and 1856 exhibited a high population growth (3% overall). Unlike some other isolated groups, they did not live long lives (expectation of life at birth was 50.4 years overall for both sexes combined), but infant mortality, at 5.5% of births is lower than might have been expected. The level of marital fertility on Pitcairn was extremely high, higher even than for the Hutterites. Although births outside marriage were uncommon, brides were frequently pregnant at marriage. Age at first marriage changed markedly in response to changes in the community. There is some evidence that premarital sex became more frequent, possibly as an adjustment to Tahitian behavioural norms within the community's English religious ethic.